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Chemische Zusammensetzung des Kapselmaterials von Staphylococcus aureus Stamm 1193/74
Author(s) -
Seltmann G.,
Beer W.
Publication year - 1976
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19760160605
Subject(s) - trichloroacetic acid , chemistry , staphylococcus aureus , polysaccharide , chromatography , ethanol precipitation , composition (language) , paper chromatography , amino acid , biochemistry , bacteria , biology , genetics , linguistics , philosophy
The capsule material of Staphylococcus aureus strain 1193/74 could be separated by precipitation with trichloroacetic acid and ethanol as well as by chromatography on DEAE‐cellulose into 13 fractions. All fractions contained saccharides and uronic acids as well as amino acids and appeared in their qualitative composition rather similar. However, in quantitative composition and in chromatographic behaviour a rather high degree of heterogeneity could be observed. No clear cut separation of protein and polysaccharide material could be achieved in any fraction. It is supposed, therefore, that the capsule material does not represent merely an acidic polysaccharide, but contains certain amounts of amino acids or peptides varying in a rather wide range.