Premium
Stoichiometry of methane oxidation in the methane‐oxidizing strain M 102 under the influence of various CH 4 /O 2 mixtures
Author(s) -
Naguib M.
Publication year - 1976
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19760160604
Subject(s) - methane , oxidizing agent , stoichiometry , oxygen , anaerobic oxidation of methane , yield (engineering) , chemistry , analytical chemistry (journal) , materials science , environmental chemistry , organic chemistry , composite material
In laboratory‐scale experiments with growing cells of the obligate methane‐oxidizing strain M 102, an overall molar gas turnover ratio of the order given below could be postulated:\documentclass{article}\pagestyle{empty}\begin{document}$$ 1{\rm CH}_{\rm 4} + 1 - 1.2{\rm O}_{\rm 2} = 0.3{\rm CO}_{\rm 2} + {\rm water}. $$\end{document}Expectations that the optimal gas mixture of methane and oxygen should lie within the range of this stoichiometric consumption ratio have been verified in fermenter 5 1 batch culture experiments. The optimal range of methane/oxygen mixture, found under the experimental conditions described, is based on the estimated growth parameters as generation and doubling times, yield coefficients related to methane and oxygen, and the efficiency of methane metabolism as indicated in the absolute amounts of CH 4 , O 2 , and CO 2 turned over. The mentioned stoichiometric relation of 1 CH 4 : 1–1.2 O 2 did not change with varying the composition, i.e. the partial pressures of CH 4 and O 2 introduced as a mixture to the cells. The efficiency of methane oxidation was obviously influenced and decreased markedly when deviating from the broad optimal range of CH 4 /O 2 mixtures. With non‐growing cells, on the other hand, the stoichiometric relation showed a considerable shift (1:1.4–1.8 CH 4 :O 2 ) with a clear tendency towards more O 2 consumption. The oxidation potential of growing cells, seems then to have a linear interdependence to the substrate concentrations, i.e. partial pressures.