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Effects of 3′‐Fluorothymidine, nalidixic acid, and mitomycin C on temperature‐sensitive mutants of the DNA polymerase of phage T4
Author(s) -
Presber H. W.,
Schroeder C.,
Rosenthal H.A.
Publication year - 1976
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19760160205
Subject(s) - nalidixic acid , dna polymerase , mutant , exonuclease , polymerase , mitomycin c , enzyme , dna polymerase ii , dna , microbiology and biotechnology , chemistry , biology , biochemistry , gene , genetics , polymerase chain reaction , escherichia coli , reverse transcriptase
Abstract The temperature‐sensitive DNA polymerase mutator mutants A58 and L98 of T4 are less inhibited by 3′‐Fluorothymidine than the T4 wild type and the antimutator mutant CB121, in consequence of the assumed differences between the polymerase‐associated exonuclease activities. This interpretation is confirmed by results with nalidixic acid and mitomycin C. The use of systems of different temperature‐sensitive mutants of one or more genes is proposed for 1.) investigating the mode of action of drugs, 2.) studies on the mechanism of enzyme action and the functions affected in temperature‐sensitive enzymes, and 3.) for enzyme‐specific drug screening.