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Wirkung von Polyen‐Makrolidantibiotica auf normale und zellwandlose Zellen von Escherichia coli W1655F +
Author(s) -
Haupt Ina,
Schuhmann Edith,
Geuther R.,
Thrum H.
Publication year - 1975
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19750150803
Subject(s) - polyene , filipin , bacteria , antibiotics , microbiology and biotechnology , escherichia coli , nystatin , neomycin , chemistry , protoplast , biology , cell wall , biochemistry , genetics , cholesterol , gene
The polyene macrolide antibiotics are active against yeasts, fungi, and other eukaryotic cells, but are with a few exceptions inactive against bacteria. The resistance of bacteria against these compounds is usually explained by the absence of sterols in their cells, the target sites of polyene antibiotics. However, in our experiments with mycotrienin, nystatin, tetramycin, lucensomycin, rimocidin, filipin, lagosin, flavofungin, flavomycoin, antibiotic 2814 P, antibiotic 5001 P, and candicidin it was demonstrated that bacteria may be susceptible to polyene antibiotics, too, if the wall‐less stable protoplast type L‐form of E. coli W1655F + is used. The measured growth inhibition concentrations were comparable with those of typical antibacterial antibiotics. Our experiments have shown that the normal rod form and the wall‐less L‐form of E. coli W1655F + contain traces of sterols in nearly the same concentration range. This means that the selective sensitivity of the L‐form cannot be explained by higher sterol content of these cells in comparison to the resistant normal rod form cells. We assume that the bacterial cell wall is responsible for the resistance of the normal rod form by masking internal target sites.