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Biosynthesis of [7‐ 3 H]16α‐hydroxy‐dehydroepiandrosterone having high specific activity
Author(s) -
Iida M.,
Matsuhashi K.,
Nakayama T.
Publication year - 1975
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19750150307
Subject(s) - dehydroepiandrosterone , hydroxylation , isotope dilution , chemistry , biosynthesis , radiochemistry , tritium , substrate (aquarium) , chromatography , nuclear chemistry , hormone , biochemistry , mass spectrometry , enzyme , biology , androgen , ecology , physics , nuclear physics
Biosynthesis of [7‐ 3 H]16α‐hydroxy‐dehydroepiandrosterone in high specific activity has been studied. [7‐ 3 H] dehydroepiandrosterone (13.9 C/mM) in trace quantity was oxidized by Streptomyces roseochromogenes (NRRLB‐1233) for 5 min at 27 °C. The radioactive products were chromatographically separated, identified and their radiochemical purity established by isotopic dilution analysis. [7‐ 3 H]16α‐hydroxy‐dehydroepiandrosterone (2.5 × 10 7 dpm) was obtained by microbial hydroxylation of substrate (1.9 × 10 9 dpm). In some cases [7‐ 3 H]5‐androstene‐3β, 16α, 17β‐triol in a small amount of radioactivity could be found at the prolonged reaction for 30 hr.