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Recombination deficient mutants of Streptococcus pyogenes K56
Author(s) -
Malke H.
Publication year - 1975
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19750150106
Subject(s) - methane sulfonate , transduction (biophysics) , mutant , mutagenesis , microbiology and biotechnology , recombination , streptococcus pyogenes , strain (injury) , biology , dna , ultraviolet light , gene , chemistry , bacteria , genetics , biochemistry , staphylococcus aureus , photochemistry , anatomy
Nitrosoguanidine mutagenesis of the Group A streptococcal strain K56 known as a good recipient in phage‐mediated transduction led to the isolation of two recombination deficient mutants showing transduction frequencies of chromosomal markers about 100‐fold lower than those of the parent strain. These mutants were sensitive to ultraviolet (UV) irradiation but had retained their ability to reactivate UV‐irradiated phage A25. They were cross‐sensitive to the killing action of nitrosoguanidine and ethyl methane sulfonate and showed severely inhibited DNA synthesis after UV radiation.