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Temperature and hydrostatic pressure effects on gelatinase activity of a Vibrio sp. and partially purified gelatinase
Author(s) -
Weimer Mary S.,
Morita R. Y.
Publication year - 1974
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19740140810
Subject(s) - gelatinase , hydrostatic pressure , fractionation , chemistry , ammonium , ammonium sulfate , chromatography , biochemistry , enzyme , physics , organic chemistry , thermodynamics
Abstract The production of gelatinase by psychrophilic Vibrio sp. and its subsequent activity were shown to be maximal at 24°C (the highest growth temperature for the organism) and pH 8.8. Hydrostatic pressure caused a decrease in cells' gelatinase production and activity. Gelatinase, partially purified by ammonium sulfate fractionation and column chromatography, demonstrated optimal activity at 40°C and pH 9.3. In the absence of NaCl, no activity was observed while optimal activity occurred at 4.0% NaCl concentration. A greater decrease in activity was caused by hydrostatic pressure at 25°C than at 40°C.