Temperature and hydrostatic pressure effects on gelatinase activity of a  Vibrio sp.  and partially purified gelatinase | Zendy

Weimer Mary S. | Zendy; Morita R. Y. | Zendy

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Temperature and hydrostatic pressure effects on gelatinase activity of a Vibrio sp. and partially purified gelatinase

Author(s) -

Weimer Mary S.,

Morita R. Y.

Publication year - 1974

Publication title -

zeitschrift für allgemeine mikrobiologie

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.58

H-Index - 54

eISSN - 1521-4028

pISSN - 0044-2208

DOI - 10.1002/jobm.19740140810

Subject(s) - gelatinase , hydrostatic pressure , fractionation , chemistry , ammonium , ammonium sulfate , chromatography , biochemistry , enzyme , physics , organic chemistry , thermodynamics

The production of gelatinase by psychrophilic Vibrio sp. and its subsequent activity were shown to be maximal at 24°C (the highest growth temperature for the organism) and pH 8.8. Hydrostatic pressure caused a decrease in cells' gelatinase production and activity. Gelatinase, partially purified by ammonium sulfate fractionation and column chromatography, demonstrated optimal activity at 40°C and pH 9.3. In the absence of NaCl, no activity was observed while optimal activity occurred at 4.0% NaCl concentration. A greater decrease in activity was caused by hydrostatic pressure at 25°C than at 40°C.

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