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Abbau von Tryptophan durch Candida guilliermondii , Stamm H17
Author(s) -
Teuscher Gisela,
Karczewski P.,
Weide H.
Publication year - 1974
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19740140508
Subject(s) - anthranilic acid , tryptamine , tryptophan , chemistry , metabolite , kynurenine , biochemistry , decarboxylation , metabolism , organic chemistry , chromatography , amino acid , catalysis
In replacementexperiments L‐tryptophan was rapidly metabolized by Candida guilliermondii , strain H17, cultivated before in a medium containing n ‐alkanes as sole carbon sources. After growth in a medium containing glucose tryptophan utilization was suppressed. Thin layer chromatography showed tryptophol as main metabolite. Kynurenine, anthranilic acid, and other substances of the aromatic pathway of tryptophan metabolism could not be detected. Feeding experiments showed a significant degradation of anthranilic acid, tryptamine, indolylpyruvic acid, and indolylacetic acid. As a metabolite of tryptamine utilization tryptophol has been identified. Degradation of formyl kynurenine and formyl anthranilic acid was within limits of error. Transamination and decarboxylation are discussed to be the primary reactions in the degradation of L‐tryptophan under the conditions used in this study.