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Nachweismethoden und Verbreitung von Pektinolyse bei Bacillus ‐Arten
Author(s) -
Steinigeweg Rosmarie,
Ottow J. C. G.
Publication year - 1974
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19740140507
Subject(s) - bacillus licheniformis , pectin , calcium , biology , chemistry , bacillus (shape) , food science , microbiology and biotechnology , bacillus subtilis , bacteria , genetics , organic chemistry
Using 4 different methods, a total of 126 Bacillus strains representing 33 different species, was examined for their ability to degrade methoxylated and demethylated pectin. The highest number of pectinolytic strains (35) was found with a newly developed, tubed polypectate‐gel, which gave complete liquefaction upon pectinolysis. The pectinolytic activity appeared to be restricted to 8 recognized species belonging to 5 groups: The B. maceranspolymyxa group, the B. licheniformis‐pumilius‐subtilis group, B. pantothenticus, B. alvei , and B. lentus. However, not all strains of a single species revealed a positive pectinolytic activity. Thus, only a part of the strains of the second and fifth group were found to be pectinolytic. Pectinolysis with B. alvei (ILM 21, JO 42) and B. lentus (CCM 35, CCM 2214) was recorded not until a prolonged incubation in the tubed pectate‐gel was used. Pectinolysis of selected B. licheniformis and B. pantothenticus strains was stimulated markedly by ammonium‐ and phosphat‐ions at various pH levels. The simultaneous addition of calcium and phosphate to the medium decreased the intensity of pectinolysis clearly. Calcium, if added solely, stimulated pectinolytic activity of B. pantothenticus strains only at a reduced concentration.