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Licht‐ und elektronenoptische Untersuchungen an Protein‐kristallen und Mikrotubuli in Hefeprotoplasten
Author(s) -
May R.,
Gottstein H.
Publication year - 1974
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19740140506
Subject(s) - microtubule , lamellar structure , crystal (programming language) , crystallography , hexagonal crystal system , electron microscope , plane (geometry) , perpendicular , materials science , optics , chemistry , physics , biology , geometry , mathematics , computer science , programming language , microbiology and biotechnology
Unusual structures ‐ crystals, crystal‐associated microtubules, and bundles of microtubules ‐ have been observed in protoplasts, but not in normally grown cells of Saccharomyces carlsbergensis and S. cerevisiae. The leaflet‐like crystals (diameter 0.3‐3 μm, thickness 0.01‐0.1 μm) occurred in the cyto‐ and karyoplasm. Crystals perpendicularly sectioned to the plane frequently showed highly regular arrays of electron‐opaque lines (periodicity 15 nm). Lamellar, rectangular, and hexagonal patterns could be seen on crystals sectioned parallelly to the plane. Smaller microtubules (outer diameter 15 nm) packed to compact bundles or fixed on crystals occurred only in the karyoplasm and they did not show any direct relations to the thicker microtubulus (outer diameter 20 nm) of the spindle apparatus. It is suggested that the crystals could be aggregations of tubulin or virus protein.