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Growth and cell division of Escherichia coli 173‐25 in the presence of some analogues of diaminopimelic acid
Author(s) -
Chaloupka J.,
Strnadová M.,
Čáslavská J.,
Vereš K.
Publication year - 1974
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19740140403
Subject(s) - diaminopimelic acid , lanthionine , dna synthesis , escherichia coli , cell division , biochemistry , thymidine , biology , mutant , dna , cell wall , chemistry , stereochemistry , microbiology and biotechnology , amino acid , cell , peptidoglycan , gene
The dap‐dependent mutant of Escherichia coli could grow in the presence of several analogues of diaminopimelic acid (dap). The multiplication proceeded without any apparent difference for several hours when dap was replaced with 2,6‐diamino‐4‐fluoro‐pimelic acid or lanthionine. Only growth without accompanying division could be detected in the presence of 2,6‐diamino‐4‐hydroxypimelic acid (hydroxy‐dap). 3 H‐hydroxy‐dap was incorporated into the TCA precipitable material under these conditions. 2,4,6‐triaminopimelic acid, probably also 2,6‐diamino‐5‐oxo‐pimelic acid could not substitute dap in the synthesis of murein. The synthesis of DNA measured by the incorporation of 3 H‐thymidine was not inhibited by hydroxy‐dap but the formation of the cross wall was impaired. The rods were longer, formed bulges in the central part of the cell and ultimately lyzed. Selenium‐lanththionine was more toxic but even in its presence some growth occurred and DNA synthesis took place. The replacement of dap with hydroxy‐dap stimulated the degradation of the wall mucopeptide during the growth.