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Electron microscopic observations of Pseudomonas aeruginosa
Author(s) -
Baechler C. A.,
Berk R. S.
Publication year - 1974
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19740140402
Subject(s) - fimbria , pseudomonas aeruginosa , microtubule , budding , pilus , biophysics , chemistry , electron microscope , bacteria , microbiology and biotechnology , materials science , crystallography , biology , escherichia coli , optics , physics , biochemistry , genetics , gene
Preparative techniques were developed for the purpose of visualizing, by negative contrast, some of the cytological characteristics of unsectioned cells of Pseudomonas aeruginosa. Concentrated fimbriae free from cells had a length of approximately 1.5 μm and a diameter of 6 nm. Pyocin‐like structures ranging in length from 32‐64 nm with a width of 8.6 nm were “attached” to the terminal ends of a small percentage of these fimbriae. Microtubules and ringlets having a diameter of 10‐20 nm were also visualized in the bacteria. Budding or evagination of cell wall material in multiple units up to 135 nm in length was frequently observed in older cultures grown on agar media.