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Monosaccharide formation from hydrocarbon by Candida rugosa
Author(s) -
Iida M.,
Finnerty W. R.
Publication year - 1974
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19740140205
Subject(s) - monosaccharide , alkane , chemistry , hydrocarbon , organic chemistry
Monosaccharide formation resulting from hydrocarbon oxidation by Candida rugosa was determined. C. rugosa was grown in a mineral medium containing 1% alkane (C 10 ‐C 17 ) as sole carbon source. Monosaccharide formation was found on all hydrocarbon substrates tested. A similar result was obtained from fatty acids as intermediate oxidation product of hydrocarbon. An interrelationship between cell growth and formation of monosaccharide was observed in all cases. The amount of monosaccharide with n ‐alkane was greater than with 1‐alkene. Also, n ‐alkanes of even‐carbon numbers yield approximately 50% more monosaccharide as compared with n ‐alkanes of odd‐carbon numbers. The optimal substrates for monosaccharide formation were n ‐dodecane and tetradecane. These yields were 170.7 and 181 μg/ml culture broth, respectively. The monosaccharide formed from n ‐alkane oxidation was D‐glucose as determined by thin‐layer chromatography, infrared spectroscopy, and nuclear magnetic resonance analyses. No extrapolysaccharide was detected in C. rugosa. The biosynthesis of extracellular saccharide is discussed.