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Aerobic inhibition of nitrate assimilation in Escherichia coli
Author(s) -
Kobayashi Miya,
Ishimoto M.
Publication year - 1973
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19730130505
Subject(s) - nitrate reductase , nitrate , nitrite , escherichia coli , ammonium , anaerobic exercise , chemistry , nitrogen assimilation , assimilation (phonology) , food science , nitrite reductase , growth medium , nitrogen , biochemistry , microbiology and biotechnology , bacteria , biology , enzyme , physiology , linguistics , philosophy , genetics , organic chemistry , gene
Growth of Escherichia coli , strain K‐10, in synthetic medium with nitrate as a sole source of nitrogen was not found under aerobic conditions, but found in the medium under anaerobic conditions as well as in synthetic medium with ammonium or nitrite under both aerobic and anaerobic conditions. The presence of nitrate reductase activity in cells indicates that the growth inhibition is due to the inhibition of the enzyme by oxygen and not due to its absence. Conditions of preculture, inoculation size, pH, and composition of the nitratesynthetic medium did not affect the inhibition. All the 37 strains of E. coli tested were grown in the nitrate medium only anaerobically, while 18 strains of Klebsiella‐Aerobacter among 22 strains tested were grown in the nitrate medium both aerobically and anaerobically.