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Comparative electron microscopic studies on Pseudomonas aeruginosa endotoxin
Author(s) -
Dyke J.,
Berk R. S.
Publication year - 1973
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19730130503
Subject(s) - trichloroacetic acid , chemistry , phenol , carbenicillin , extraction (chemistry) , lysozyme , staining , chromatography , pseudomonas aeruginosa , aqueous solution , pseudomonas , microbiology and biotechnology , bacteria , antibiotics , biochemistry , biology , gentamicin , organic chemistry , genetics
The endotoxin from Pseudomonas aeruginosa C 9 was obtained by five standard methods of extraction for comparative electron microscopic studies. Observation of the five preparations demonstrated the presence of two major types of structures. The first of these was seen in endotoxins prepared by trichloroacetic acid, ethyl ether, hot water, and ethylenediamineteraacetate‐lysozyme extraction, and consisted of discrete spherules containing smaller spherules within and having a homogeneous staining centre and rod‐like border. The other morphologic type was seen only in preparations obtained by the aqueous phenol technique and consisted of pleomorphic staining material and rodlets. Preparations isolated by trichloroacetic acid extraction by phenol extraction. Loss in structural integrity was encountered upon exposure to polymxin B, colistin sulfate and carbenicillin, but not with other antibiotics tested.