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Comparative studies on Pseudomonas aeruginosa endotoxin
Author(s) -
Dyke J. W.,
Berk R. S.
Publication year - 1973
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19730130404
Subject(s) - trichloroacetic acid , chemistry , phenol , extraction (chemistry) , chromatography , aqueous solution , pseudomonas aeruginosa , lysozyme , hydroxylamine , lethal dose , biochemistry , bacteria , biology , organic chemistry , genetics
Attempts to obtain toxic preparations of endotoxin from Pseudomonas aeruginosa (C 2 ) were initiated by employing five different extraction procedures which were subsequently assayed for mouse lethality using a variety of routes. The standard methods of extraction were the aqueous phenol, trichloracetic acid, ethylenediaminetetraacetate‐lysozyme, ethyl ether, and hot water procedures. The aqueous phenol preparation was found to be the most toxic and exhibited an LD 50 value of 450 μg dry weight when administered intravenously and 840 μg intraperitoneally. No lethality was observed when endotoxin was administered intranasally. The second most lethal preparation was obtained by the trichloroacetic acid extraction and yielded LD 50 values of 589 μg intravenously and 947 μg intraperitoneally. The other three preparations were considerably less lethal. The aqueous phenol and trichloroacetic acid preparation tended to have a higher content of those carbohydrates associated with the core region of the lipopolyssacharide molecule than did the lesser toxic preparations. Some correlation between lethality and lipid content as determined by the alkaline hydroxylamine procedure was also observed.