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Physiologie der Nitratreduktion bei Pseudomonas aeruginosa
Author(s) -
Tan T. L.
Publication year - 1973
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19730130108
Subject(s) - nitrate , nitrite , ammonium , chemistry , nitrogen , strain (injury) , bacteria , nutrient , ammonium nitrate , denitrification , food science , nuclear chemistry , biology , organic chemistry , genetics , anatomy
By both Pseudomonas strains the N 2 and N 2 O production depended on the “physiological condition” of the bacteria. The N 2 O formation by strain C 31 in mineral media depended also on the precultivation medium. The bacteria produced N 2 O besides N 2 , if precultivated in Nutrient Broth + KNO 3 . Precultivated in a mineral medium, however, they produced only N 2 . The anaerobic gas production of Pseudomonas aeruginosa strain C 31 and strain N.C.I.B 8704 was determined under hydrogen atmosphere. The results are as follows: In Nutrient Broth + 0.35% KNO 3 nitrate was respirated quantitatively to N 2 and N 2 O by the two strains. On the contrary, in Nutrient Broth + 0.5% KNO 3 nitrate was not completely consumed by strain C 31. In nitrate‐ammonium‐medium we succeeded to recover quantitatively the given nitrogen compounds (nitrate, nitrite, ammonium, organic N), partially in the denitrification products, after the end of the experiment, but not in the experiment with nitrate‐medium. In nitrate‐medium strain C 31 produced a much higher amount of nitrite (up to 73 mg N/l) as in nitrate‐ammonium‐medium (up to 213 μg N/l). The influence of the ammonium ions is discussed.