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Einfluß von Purin‐ und Pyrimidinderivaten auf die Prophageninduktion in Escherichia coli C600 T44
Author(s) -
Noack D.,
Klaus S.
Publication year - 1972
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19720120708
Subject(s) - lysogenic cycle , prophage , chemistry , escherichia coli , nucleic acid metabolism , nucleic acid , guanosine , microbiology and biotechnology , chloramphenicol , strain (injury) , biochemistry , biology , antibiotics , bacteriophage , rna , gene , anatomy
The inducibility of the prophages λ + and λ ind 8 within the lysogenic, temperature sensitive strain E. coli C600 T44 can be influenced by precursors of nucleic acid metabolism or by substances chemically related to those. With an agar diffusion test 63 substances of this kind were tested both qualitatively and quantitatively. It was shown that there are substances with stimulating or inhibiting action, respectively, on the inducibility of prophages. Furthermore, the prophages λ + and λ ind s exhibit different reactions on the exposure of the respective lysogenic strains against some substances tested. A small selection of effective substances are moreover qualitatively tested on the nonlysogenic strain E. coli C600 T44 and on the lysogenic complex E. coli C600 T44 ( λ ind ‐ ) the prophage of which is known to be not inducible by UV light. The results are discussed in terms of a different sensitivity of the lambda phage repressors against intermediates of the nucleic acid metabolism. An immediate comparison between the action of test substances and control substances such as adenine. guanosine, chloramphenicol, and nalidixic acid is possible on each test plate.