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Synthesis of inducible phospholipase A with antibacterial activity by Bacillus cereus
Author(s) -
Gaál Vera,
Ivánovics G.
Publication year - 1972
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19720120506
Subject(s) - bacillus cereus , bacteriocin , bacillus megaterium , lysis , microbiology and biotechnology , antibacterial activity , phospholipase a , bacillus (shape) , phospholipase , biology , liberation , antibacterial agent , bacteria , phospholipase a2 , biochemistry , antibiotics , antimicrobial , enzyme , in vitro , genetics
Mitomycin C was added to cultures of Bacillus cereus growing exponentially. Mass lysis in cultures of all strains (altogether 12) ensued at reincubation was associated with liberation either of an antibacterial substance or a phage; lysates of two strains (W and V8) contained both phage and bacteriocin of high titre. Several characteristics of the antibacterial substance, released by these two strains, appeared to be identical with those of megacin A, a bacteriocin of Bacillus megaterium. Analysis of a homomolecular fraction of a purified active material resolved by gel electrophoresis suggested that the antibacterial substance is identical with phospholipase A.