Premium
Autotrophic growth of hydrogen bacteria in continuous culture
Author(s) -
Voytovich J. V.,
Gitelson I. I.,
Ponomaryev P. I.,
Sidko F. Ya.,
Terskov I. A.,
Trubachov I. N.
Publication year - 2007
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19720120111
Subject(s) - bacteria , hydrogen , carbon dioxide , oxygen , biomass (ecology) , nitrogen , chemistry , amino acid , autotroph , food science , biochemistry , biology , environmental chemistry , organic chemistry , ecology , genetics
A laboratory installation was developed for continuous cultivation of hydrogen bacteria by turbidistate with a capacity of about 5 1. A steady state process with Hydrogenomonas eutropha was realized which produces up to 40 g/1 · day of dry biomass. Optimum concentration values for oxygen, hydrogen, carbon dioxide in the gas phase for various densities of bacterial suspensions were found. The minimum concentrations of bioelements are: N 50–70 mg/1, P, Mg, K 20–40 mg/1. The biomass of hydrogen bacteria is characterized by a high protein content (up to 77%) including irreplaceable amino acids up to 40%, and nucleic acids up to 10%. Oxygen and nitrogen deficiency lead to a protein decrease up to 46% and an increase in the concentration of poly‐β‐hydroxybutyric acid. The possibility of a continuous unsterile cultivation of Hydrogenomonas with prevalence over the growth of the accompanying microflora is shown.