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Biochemical analysis of bacterial macromutations
Author(s) -
Roe C. R.,
You K. S.
Publication year - 1971
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19710110607
Subject(s) - cytosine , mutant , dna , guanine , gc content , biochemistry , thymine , chemistry , biology , nucleotide , gene , genome
A mutant (DNA GC 70%) of Pseudomonas testosteroni (DNA GC 60%) has been shown to produce new strains of P. testosteroni having the same GC content as the parent strain 78 (60 moles %). Analyses of the protein and analogous enzymes by electrophoresis reveal an acidic alteration in the charge of all soluble proteins. Evaluation of the organisms in the Bacillus and Paracolon systems clearly demonstrates that total protein charge alterations from acidic to basic or basic to acidic are common features associated with alterations of DNA base compositions. The reported DNA content changes of mutants appears to follow a rule of single or multiple 25% substitution of parental adenine‐thymine content to guanine‐cytosine content in the mutant produced. The accuracy of this prediction of such a percentage change in mutant DNA content is presented. The common features of extent of GC alteration and protein charge changes observed in the Pseudomonas, Bacillus , and Paracolon systems suggest the possibility of a single mutational event producing an organism which is classified in a genus other then the parent.