Die autoradiographische Lokalisation der DNS in thyminbedürftigen Zellen von Escherichia coli CR 34 nach Thyminmangel

Under conditions of thymine starvation the cells of the thyminerequiring strain E. coli CR 34 elongate and give rise to filamentous forms without septa. In 95% of the cells the 3 H‐thymidine labelled material is characteristically confined to the center of the cells and does not extend out to the tips of the filaments. With different methods it is shown that a proportion of the cells still may divide once when DNA synthesis is inhibited by thymine depletion. It is suggested, that a filament formed under conditions of thymine starvation mostly contains one „nucleus”.