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Die autoradiographische Lokalisation der DNS in thyminbedürftigen Zellen von Escherichia coli CR 34 nach Thyminmangel
Author(s) -
Strohbach G.
Publication year - 1969
Publication title -
zeitschrift für allgemeine mikrobiologie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0044-2208
DOI - 10.1002/jobm.19690090607
Subject(s) - thymine , escherichia coli , chemistry , thymidine , dna synthesis , dna , strain (injury) , microbiology and biotechnology , biology , biochemistry , anatomy , gene
Abstract Under conditions of thymine starvation the cells of the thyminerequiring strain E. coli CR 34 elongate and give rise to filamentous forms without septa. In 95% of the cells the 3 H‐thymidine labelled material is characteristically confined to the center of the cells and does not extend out to the tips of the filaments. With different methods it is shown that a proportion of the cells still may divide once when DNA synthesis is inhibited by thymine depletion. It is suggested, that a filament formed under conditions of thymine starvation mostly contains one „nucleus”.