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Immediate early gene c‐ fos regulates the synthesis of phospholipids but not of gangliosides
Author(s) -
Guido M. E.,
de Arriba Zerpa G. A.,
Bussolino D. F.,
Caputto B. L.
Publication year - 1996
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490430112
Subject(s) - stimulation , immediate early gene , gene , ganglioside , biology , protein biosynthesis , phospholipid , in vivo , microbiology and biotechnology , gene expression , biochemistry , cell , membrane , neuroscience , genetics
Retinal ganglion cells isolated from chicks that in vivo were exposed to light have a higher phospholipid labeling capacity than those obtained from animals in the dark. Actinomycin D or a mixture of protein synthesis inhibitors or of antisense oligonucleotides to c fos plus c‐jun injected intraocularly 1 hr prior to the stimulation period, abolished the light‐dark differences for phospholipids but not for gangliosides. Light stimulation induced the formation (and/or stabilization) of c fos mRNA and of the protein c‐Fos, indicating that immediate early gene induction, and consequently the synthesis of the protein(s) encoded, is essential to increase the synthesis of phospholipids but not of gangliosides. These results suggest a novel mechanism by which immediate early genes engram neural cells, modifying specifically the metabolism of cell constituents producing long‐lasting changes in the cells. © 1996 Wiley‐Liss, Inc.