Premium
Regulation of the rat S100β gene expression: The role of the 2 kb 5′‐upstream sequence in glial specific expression
Author(s) -
Hagiwara N.,
Sueoka N.
Publication year - 1995
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490420502
Subject(s) - exon , biology , cell culture , transfection , microbiology and biotechnology , cell type , gene , luciferase , gene expression , upstream activating sequence , cell , genetics , promoter
We have studied the role of the 2047 by 5′‐upstream region and 232 by first exon sequence of the rat S100β gene in glial specific expression. S100β luciferase expression vectors carrying serial deletions of the S100β 5′; upstream sequence were constructed and transiently transfected into the peripheral nervous system (PNS) glial‐type cell line RT4‐D6, the PNS neuronal‐type cell line RT4‐E5, and the central nervous system (CNS) glial‐type cell line C6. The hepatoma cell line HTC was also transfected as a non‐neural control. From this functional analysis, we found a glial‐specific positive regulatory sequence(s) mapped between −583 and −106 relative to the transcriptional start site. This region confers a significantly higher level of luciferase expression in the glial‐type cell lines RT4‐D6 and C6 than in the neuronal cell line RT4‐E5 and the hepatoma cell line HTC. Also, a non‐cell type specific positive regulatory element was identified in the first exon sequence between + 78 and + 232. Though non‐cell type‐specific, it was found to have a predominant effect in glial cells. From these observations, we have concluded that the 2047 by 5′;‐upstream region and 232 by of the first exon sequence confers the high levels of S100β expression in glial cells through these two positive elements. © 1995 Wiley‐Liss, Inc.