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Zebrafish neurons express two L1‐related molecules during early axonogenesis
Author(s) -
Tongiorgi E.,
Bernhardt R. R.,
Schachner M.
Publication year - 1995
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490420413
Subject(s) - zebrafish , hindbrain , biology , in situ hybridization , rhombomere , neuroscience , spinal cord , microbiology and biotechnology , nervous system , embryonic stem cell , central nervous system , gene , gene expression , genetics , hox gene
Partial clones coding for two L1‐related genes, zebrafish L1.1 and L1.2, were isolated from a zebrafish embryonic cDNA library. The homology analysis, based on the deduced amino acid sequences of L1.1 and L1.2, revealed that the two molecules are most closely related to each other and to mouse L1. Analysis by in situ hybridization revealed that during embryonic development of the nervous system the L1.1 and L1.2 messages are restricted to postmitotic neurons and that the onset of expression correlates with the initiation of axonogenesis. L1.1 is expressed by all known classes of neurons, consistent with an important general function during axonal outgrowth. Most of the neurons also express L1.2. However, L1.2 either is undetectable or is expressed at very low levels in the neurons of the olfactory placodes, anterior lateral line/acoustic ganglia complex, posterior lateral line ganglion, and in late developing hindbrain neurons. In the spinal cord, L1.2 message is detected only in a subpopulation of Rohon‐Beard cells. This suggests the possibility that different levels of L1.2 expression may serve to distinguish different populations of neurons and their axons. © 1995 Wiley‐Liss, Inc.