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Membrane properties of cultured glial cells obtained mechanically or by enzymatic dissociation: Differential alterations by gangliosides
Author(s) -
Bouchon B.,
Petegnief V.,
Rebel G.
Publication year - 1995
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490410410
Subject(s) - ganglioside , enzyme , dissociation (chemistry) , membrane , chemistry , biochemistry , biophysics , taurine , microbiology and biotechnology , biology , amino acid
Rat astroglial cells were obtained either mechanically or by enzymatic dissociation and some properties of the primary and secondary cultures were studied. Differences in the ganglioside amount, taurine uptake, membrane fluorescence anisotropy, or their respective modulation by total brain gangliosides confirmed that although the two types of cultures are morphologically similar, they are biochemically distinct. © 1995 Wiley‐Liss, Inc.