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Myelin glycoprotein P 0 is expressed at early stages of chicken and rat embryogenesis
Author(s) -
Zhang S.M.,
Marsh R.,
Ratner N.,
Brackenbury Robert
Publication year - 1995
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490400213
Subject(s) - epitope , embryo , messenger rna , biology , monoclonal antibody , microbiology and biotechnology , myelin associated glycoprotein , western blot , glycoprotein , embryogenesis , myelin basic protein , myelin , antigen , antibody , immunology , gene , biochemistry , neuroscience , central nervous system
Although previous studies suggest that P 0 is expressed only in myelinating Schwann cells, monoclonal antibody 1E8 reacts with P 0 , yet also stains early Schwann cell precursors and non‐myelinating Schwann cells (Bhattacharyya et al.: Neuron 7:831–844, 1991). We therefore characterized the 1E8 epitope and analyzed P 0 mRNA expression during development. Immunoblot analyses of P 0 fusion proteins and of deglycosylated P 0 indicated that the 1E8 epitope is polypeptide. Northern blot and polymerase chain reaction (PCR) analyses revealed that P 0 is encoded by a single mRNA that is expressed in chicken embryos as early as E4 and in rat embryos as early as E14. These data indicate that the antigen recognized by 1E8 in early chicken embryos is P 0 and that, during development of both chickens and rats, P 0 mRNA is expressed long before myelination. © 1995 Wiley‐Liss, Inc.