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Nerve growth factor induces the Oct‐2 transcription factor in sensory neurons with the kinetics of an immediate‐early gene
Author(s) -
Kendall G.,
BrarRai A.,
Ensor E.,
Winter J.,
Wood J. N.,
Latchman D. S.
Publication year - 1995
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490400205
Subject(s) - nerve growth factor , cycloheximide , dorsal root ganglion , transcription factor , biology , neurotrophin , microbiology and biotechnology , messenger rna , immediate early gene , gene expression , fibroblast growth factor , growth factor , neurotrophic factors , sensory neuron , endocrinology , medicine , gene , neuroscience , protein biosynthesis , sensory system , genetics , receptor
The Oct‐2 transcription factor has a predominantly inhibitory effect on gene expression in neuronal cell lines. This factor and its corresponding mRNA have previously been shown to be elevated in adult rat dorsal root ganglion (DRG) neurons chronically exposed to nerve growth factor (NGF). Here we show that the Oct‐2 mRNA is rapidly induced in DRG cells exposed to NGF and that such induction still occurs to a lesser extent in the presence of the protein synthesis inhibitor cycloheximide. These findings characterize Oct‐2 as a novel member of the immediate‐early class of NGF‐induced transcription factors whose previously defined members have a predominantly stimulatory effect on the expression of other genes. Induction of the Oct‐2 mRNA was also observed in DRG neurons treated with acidic fibroblast growth factor or epidermal growth factor but not with a range of other growth factors and neurotrophins. The role of the Oct‐2 transcription factor in mediating the response of DRG neurons to NGF is discussed. © 1995 Wiley‐Liss, Inc.