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Partial purification of a novel mitogen for oligodendroglia
Author(s) -
Kreider Barbara Q.,
Grinspan J. B.,
Waterstone M. B.,
Bramblett G. T.,
Ances B.,
Williams M.,
Stern J.,
Lee V. M.Y.,
Pleasure D.
Publication year - 1995
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490400106
Subject(s) - progenitor cell , growth factor , platelet derived growth factor , basic fibroblast growth factor , platelet derived growth factor receptor , biology , fibroblast growth factor , microbiology and biotechnology , mitogen activated protein kinase , chemistry , biochemistry , stem cell , kinase , receptor
A protein with a MW app of 50–70 kDa isolated from the salt extract of crude membranes from neonatal rat brain increases the numbers of oligodendroglia in mixed glial cultures prepared from neonatal rat cerebral white matter. After partial purification by ion exchange and gel exclusion chromatography, and elution from an SDS‐polyacrylamide gel, this protein ( “oligodendroglial trophic factor,” OTF) elicited half‐maximal oligodendroglial recruitment at a concentration of 5 ng/mL. OTF is a mitogen for oligodendroglia, and to a lesser extent, for oligodendroglial progenitor (O2A) cells, but does not stimulate proliferation of astroglia, Schwann cells, or endoneurial fibroblasts. OTF, unlike platelet‐derived growth factor (PDGF), is not an oligodendroglial survival factor. Antibodies against PDGF and basic fibroblast growth factor (bFGF) do not interfere with the accumulation of oligodendroglia induced by OTF. When OTF is given simultaneously with either PDGF or bFGF, there is an additive increase in the numbers of cells of the oligodendroglial lineage. © 1995 Wiley‐Liss, Inc.