Premium
Mitogenic effects of platelet‐derived growth factor, fibroblast growth factor, transforming growth factor‐β, and heparin‐binding serum factor for adult mouse Schwann cells
Author(s) -
Watabe K.,
Fukuda T.,
Tanaka J.,
Toyohara K.,
Sakai O.
Publication year - 1994
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490390504
Subject(s) - growth factor , platelet derived growth factor receptor , forskolin , platelet derived growth factor , fibroblast growth factor , basic fibroblast growth factor , transforming growth factor , biology , bromodeoxyuridine , endocrinology , transforming growth factor beta , medicine , transforming growth factor, beta 3 , fibroblast , microbiology and biotechnology , cell culture , cell growth , tgf alpha , biochemistry , receptor , genetics , stimulation
Mitogenic effects of fetal calf serum (FCS), platelet‐derived growth factor (PDGF), fibroblast growth factor (FGF), transforming growth factor‐β (TGF‐β), and forskolin to adult mouse Schwann cells were examined by bromodeoxyuridine (BrdU) incorporation and double immunofluorescence for S100 and BrdU. PDGF‐BB, basic FGF, and TGF‐β1 and β2 were all mitogenic for Schwann cells in media containing FCS. Forskolin suppressed the mitogenic activity of these factors. In serum‐free media, PDGF‐BB and bFGF were also mitogenic, but TGF‐β1 and β2 were not. Heparin‐binding fractions of FCS obtained by heparin‐Sepharose chromatography synergized with TGF‐β1 and β2 to produce a mitogenic response. Since PDGF‐BB, acidic FGF, and basic FGF were not detected in these fractions by immunoabsorption and immunoblot assays, the presence of unidentified heparin‐binding molecules in FCS bioactive for adult mouse Schwann cells is suggested. © 1994 Wiley‐Liss, Inc.