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Neuronal progenitors identified by their inability to express class I histocompatibility antigens in response to interferon‐γ
Author(s) -
Bailey K. A.,
Drago J.,
Bartlett P. F.
Publication year - 1994
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490390207
Subject(s) - neuroepithelial cell , biology , antigen , embryonic stem cell , major histocompatibility complex , progenitor cell , cell sorting , microbiology and biotechnology , immunology , population , lineage markers , interferon gamma , interferon , flow cytometry , stem cell , genetics , cytokine , neural stem cell , medicine , gene , environmental health
Interferon‐γ (IFN‐γ) can induce class I major histocompatibility complex (MHC) antigen (H‐2) expression on virtually all neuroepithelial cells isolated from embryonic day 9 (E9) mice. However, a subpopulation of cells become refractory to H‐2 induction (H‐2l − ) by E10 and the percentage of H‐2 noninducible cells increases during development. Cell sorting, by flow cytometry or magnetic bead immunoselection, has shown that H‐2l − cells give rise exclusively to neuronal cells, and by E12, the majority of the neuronal progenitors are found within this population. It has also been found that 98% of the H‐2l − also express the neuron‐associated marker, A2B5. Cells of the glial cell lineage retain the ability to express class I antigens throughout development. From these studies, it is clear that the neuroepithelium contains cells committed to the neuronal cell lineage as early as E10 in the mouse. Copyright © 1994 Wiley‐Liss, Inc.