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Actin‐Binding protein, drebrin, accumulates in submembranous regions in parallel with neuronal differentiation
Author(s) -
Asada H.,
Uyemura K.,
Shirao T.
Publication year - 1994
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490380205
Subject(s) - cytochalasin d , cytoplasm , actin , microbiology and biotechnology , cytoskeleton , actin binding protein , cytochalasin , chemistry , actin cytoskeleton , biology , biochemistry , cell
Drebrins are developmentally regulated actin‐binding proteins. In this study, we analyzed subcellular distribution of drebrin E in neuroblastoma cells (SHSY5Y) in culture, especially in terms of its relationship to actin filaments. In undifferentiated cells, drebrin E was scattered as flocculus small dots along the stress fibers and also accumulated at adhesion plaques. In parallel with the neuronal differentiation following retinoic acid treatment, drebrin E was accumulated, accompanying filamentous (F) actin, in the submembranous cortical cytoplasm. Similar submembranous localization of drebrins was observed in primary cultured neurons. In the presence of drebrin E F‐actin was more stable against cytochalasin D than F‐actin lacking drebrin E. These results suggest that drebrin E plays a role in neuronal morphological differentiation by changing its subcellular localization with stabilized F‐actin. © 1994 Wiley‐Liss, Inc.