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Dibutyryl cAMP, interleukin‐1β, and macrophage conditioned medium enhance the ability of astrocytes to promote neurite growth
Author(s) -
Miller C.,
Tsatas O.,
David S.
Publication year - 1994
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490380108
Subject(s) - neurite , astrocyte , microbiology and biotechnology , macrophage , in vivo , in vitro , neuroscience , biology , chemistry , immunology , central nervous system , biochemistry
Astrocytes purified from the neonatal rat brain were cultured for up to 3 weeks prior to being treated with agents that can induce reactive changes in astrocytes. These agents included dibutyryl cAMP, interleukin‐1β, and macrophage conditioned media. After treating astrocytes for 3 days, the agents were removed and the ability of the astrocytes to support neurite growth was assessed by plating neonatal rat cerebellar cortical neurons. All the agents increased the ability of astrocytes to support the growth of long neurites. This was particularly evident with astrocytes treated with dibutyryl cAMP and LPS macrophageconditioned medium. These results provide direct evidence that the reactive changes induced in astrocytes in this in vitro model might aid the growth of neurites. Similar changes may underlie the injury‐induced axonal sprouting that occurs in vivo. © 1994 Wiley‐Liss, Inc.