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Extracellular ATP‐induced currents in astrocytes: Involvement of a cation channel
Author(s) -
Walz W.,
Gimpl G.,
Ohlemeyer C.,
Kettenmann H.
Publication year - 1994
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490380104
Subject(s) - extracellular , biophysics , intracellular , reversal potential , depolarization , membrane potential , chemistry , gap junction , hyperpolarization (physics) , patch clamp , biochemistry , biology , receptor , stereochemistry , nuclear magnetic resonance spectroscopy
Whole‐cell currents were measured with the perforated patch clamp technique in cultured rat astrocytes to analyze the underlying ionic mechanism for a P 2 ‐purinoceptor‐mediated depolarization. ATP (100 μM) induced an inward current with a mean amplitude of 130 pA and an EC 50 of 17 μM. The response desensitized during a 1 min application. Replacement of extracellular Na + with NMDG or K + abolished the ATP‐evoked inward current. Replacement of Na + with choline, however, resulted in an ATP‐evoked response of one‐third the amplitude in normal solution. This is indicative of a cation rather than Na + channel. However, due to difficulties in voltage‐clamping these gap junction‐coupled cells at voltages different from the membrane resting potential, the current reversal potential could not be determined. Measurements with K + ‐sensitive microelectrodes showed that 100 μM ATP lowered the intracellular K + concentration. Replacement of extracellular Ca 2+ or Cl − did not alter the ATP‐induced inward currents. Fura‐2 imaging experiments revealed a transient rise of the intracellular Ca 2+ concentration during ATP application. Removal of extracellular Ca 2+ did not influence the peak response; it did, however, shorten the time course. These results and previous observations that the permeability changes are caused by a P 2× receptor are indicative of an ATP‐sensitive cation conductance. In addition, cytoplasmic Ca 2+ is increased by mobilization from intracellular stores, and by additional influx across the cell membrane. Extracellular ATP released by neurons could evoke K + release from astrocytes as well as be a mediator for cation changes that signal cell activation processes when released by damaged cells. © 1994 Wiley‐Liss, Inc.