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Molecular cloning and expression of a δ‐opioid recetpor from rat brain
Author(s) -
Abood M. E.,
Noel M. A.,
Farnsworth J. S.,
Tao Q.
Publication year - 1994
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490370605
Subject(s) - δ opioid receptor , opioid receptor , complementary dna , opioid , receptor , clone (java method) , amino acid , molecular cloning , microbiology and biotechnology , cloning (programming) , opioid peptide , chemistry , messenger rna , biology , gene , biochemistry , computer science , programming language
We have isolated and characterized a rat δ‐opioid receptor. The deduced amino acid sequence (372 aa) closely resembles the murine δ‐opioid receptor, DOR‐1. In fact, 97% of the amino acid residues are conserved between the two species, while 93% of the nucleic acid residues are identical. A 6 kb mRNA was detected in rat cortex using rat DOR‐1 as a probe. When expressed in COS cells, the clone shows high‐affinity opioid binding with selectivity for δ‐opioids. The rat δ‐opioid receptor cDNA clone will be a useful tool for studying the function of δ‐opioid receptor in rats. Wiley‐Liss, Inc.