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Myelin basic protein content of aggregating rat brain cell cultures treated with cytokines and/or demyelinating antibody: Effects of macrophage enrichment
Author(s) -
Loughlin A. J.,
Woodroofe M. N.,
Cuzner M. L.,
Honegger P.,
Comte V.,
Matthieu J. M.
Publication year - 1994
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490370512
Subject(s) - myelin , myelin basic protein , immunocytochemistry , macrophage , myelin oligodendrocyte glycoprotein , tumor necrosis factor alpha , oligodendrocyte , immunology , cell culture , biology , cytokine , central nervous system , biochemistry , neuroscience , endocrinology , in vitro , genetics
Abstract The demyelinative potential of the cytokines interleukin‐1α (IL‐1α), interferon‐γ (IFN‐γ), and tumor necrosis factor‐α (TNF‐α) has been investigated in myelinating aggregate brain cell cultures. Treatment of myelinated cultures with these cytokines resulted in a reduction in myelin basic protein (MBP) content. This effect was additively increased by anti‐myelin/oligodendrocyte glycoprotein (α‐MOG) in the presence of complement. Qualitative immunocytochemistry demonstrated that peritoneal macrophages, added to the fetal telencephalon cell suspensions at the start of the culture period, successfully integrated into aggregate cultures. Supplementing the macrophage component of the cultures in this fashion resulted in increased accumulation of MBP. The effect of IFN‐γ on MBP content of cultures was not affected by the presence of macrophages in increased numbers. © 1994 Wiley‐Liss, Inc.