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Glucocorticoid‐induced upregulation of proteolipid protein and myelin‐associated glycoprotein genes in C6 cells
Author(s) -
Zhu W.,
Wiggins R. C.,
Konat G. W.
Publication year - 1994
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490370206
Subject(s) - retinoic acid , gene , dexamethasone , gene expression , glucocorticoid , myelin associated glycoprotein , downregulation and upregulation , northern blot , proteolipid protein 1 , biology , microbiology and biotechnology , myelin proteolipid protein , myelin , messenger rna , endocrinology , myelin basic protein , biochemistry , central nervous system
The effect of dexamethasone on the expression of proteolipid protein (PLP) and myelin‐associated glycoprotein (MAG) genes was investigated in rat C6 glioma cells. The steady state level of the respective mRNA was quantitated by Northern blot analysis. The treatment of cells with dexamethasone transiently upregulated the expression of both genes with peak mRNA levels of approximately 10‐fold over control levels occurring at day 3 for the PLP gene and at day 5 for the MAG gene. The effect was directly related to the drug concentration in the range from 10 −9 to 10 −5 M. Combined exposure of the cells to dexamethasone and retinoic acid featured an additive effect on PLP gene expression, whereas MAG gene expression was depressed below detectability level. The dissimilarity in the response of the genes to dexamethasone and retinoic acid supports the contention that the genes are controlled by different mechanisms. Furthermore, the results indicate that the effects of dexamethasone and retinoic acid on the myelin genes are mediated by different regulatory pathways. © 1994 Wiley‐Liss, Inc.