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Platelet‐activating factor is a messenger in the electroconvulsive shock‐induced transcriptional activation of c‐ fos and zif ‐268 in hippocampus
Author(s) -
Marcheselli V. L.,
Bazan N. G.
Publication year - 1994
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490370108
Subject(s) - hippocampus , chemistry , second messenger system , intracellular , platelet activating factor , synaptic plasticity , messenger rna , receptor , immediate early gene , medicine , endocrinology , gene expression , pharmacology , biology , biochemistry , gene
Platelet‐activating factor (PAF, 1‐ O ‐alkyl‐2‐acetyl‐ sn ‐glycero‐3‐phosphocholine), undetectable in resting neural tissue, accumulates in brain during seizures. A hetrazepine, BN‐50730, is shown here to displace [ 3 H]PAF‐specific binding from microsomal, but not from synaptosomal membranes, indicating selectivity for a high affinity intracellular binding site. Rats pretreated with BN‐50730 by intraperitoneal or intracerebroventricular injection exhibited an inhibition of the electroconvulsive shock (ECS)‐induced expression of c‐ fos and zif ‐268 in hippocampus. A much more pronounced, dose‐dependent inhibition of ECS‐induced zif ‐268 mRNA in hippocampus by intracerebroventricular injection of of BN‐50730 was observed. It is concluded that, in the hippocampus, PAF IS A MEDIATOR OF THE EXPRESSION OF zif ‐268 and, to a lesser extent, c‐ fos through an intracellular specific binding site. Thus, PAF may be a messenger in signal regulated zinc‐finger transcription factors, and in other immediate‐early genes involved in long‐term synaptic plasticity changes. © 1994 Wiley‐Liss, Inc.