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Characterization of the cis ‐acting elements of the mouse myelin P 2 promoter
Author(s) -
Bharucha V. A.,
Peden K. W. C.,
Subach B. R.,
Narayanan V.,
Tennekoon G. I.
Publication year - 1993
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490360503
Subject(s) - microbiology and biotechnology , enhancer , biology , transfection , myelin , promoter , gene , transcription (linguistics) , transcription factor , mutant , regulatory sequence , reporter gene , dna binding protein , fatty acid binding protein , upstream activating sequence , gene expression , genetics , central nervous system , linguistics , philosophy , neuroscience
Myelin P 2 is a basic protein of an apparent molecular weight of 14,800. Expression of P 2 has been found largely in the cytosol of Schwann cells in the peripheral nervous system. Although the function of P 2 is unknown, its striking homology to a family of fatty acid binding proteins has led to the idea that P 2 may function as a fatty acid transport molecule. To investigate the DNA elements that control the expression of P 2 , sequences 5′ to the coding region were cloned upstream of the cat reporter gene. A series of 3′ and 5′ promoter mutants was constructed and their activity determined following transfection into secondary Schwann cells and the MT 4 H1 Schwann cell line. Using this strategy, we have identified a 217 bp silencer region and a 142 bp positive regulatory region. In addition, we have localized the 5′ flanking sequences in the promoter that are responsive to cAMP induction and to the transcription factor CCAAT/enhancer binding protein (C/EBP). © 1993 Wiley‐Liss, Inc.