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Glycerophosphorylcholine phosphocholine phosphodiesterase activity during the differentiation of glial progenitor cells
Author(s) -
Monge M.,
Yuan J.,
Cabon F.,
Zalc B.,
Kanfer J. N.
Publication year - 1993
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490360410
Subject(s) - phosphocholine , phosphodiesterase , choline , progenitor cell , biology , cell culture , population , oligodendrocyte , biochemistry , microbiology and biotechnology , myelin , endocrinology , stem cell , enzyme , phospholipid , central nervous system , membrane , phosphatidylcholine , medicine , genetics , environmental health
O‐2A progenitor cells were grown in medium containing either 1% or 10% fetal calf serum (FCS) for 4 weeks. The cells in 1% FCS were 75% oligodendrocytes by 3 weeks in culture. The cell population was so overgrown with astrocytes in the 10% medium that an accurate estimate of cell number could not be made. The activities of glycerophosphorylcholine phosphocholine phosphodiesterase (GPC‐PC‐PdE), p‐nitrophenylphosphorylcholine phosphodiesterase (pNPPC‐PC‐PdE), and ceramide UDP galactose galactosyl transferase (CGalT) were barely detectable in the cells grown in 10% FCS. The activities of these 3 enzymes were low in the cells grown in 1% FCS for the first 2 weeks and then all 3 increased manyfold. These observations reinforce the evidence previously accrued showing that these two phosphodiesterase activities (GPC‐PC‐PdE and pNPPC‐PC‐PdE) are markers of oligodendroglial cells as well as myelin. In contrast, glycerophosphorylcholine choline phosphodiesterase (GPC‐C‐PdE) activities were present in cells grown in both 1% and 10% FCS. © 1993 Wiley‐Liss, Inc.