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Atypical localization of the oligodendrocytic isoform (PI) of glutathione‐S‐transferase in astrocytes during cuprizone intoxication
Author(s) -
Cammer W.,
Zhang H.
Publication year - 1993
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490360208
Subject(s) - oligodendrocyte , gliosis , neuroglia , astrocyte , biology , carbonic anhydrase , carbonic anhydrase ii , glial fibrillary acidic protein , forebrain , gene isoform , immunocytochemistry , endocrinology , myelin , biochemistry , central nervous system , neuroscience , enzyme , immunohistochemistry , immunology , gene
Immunocytochemical staining for the Pi and Mu isoforms of glutathione‐S‐transferase was used to investigate changes in the glial cells in the mouse forebrain. During early development in mouse forebrains the localizations of carbonic anhydrase, Pi and Mu were similar to the respective cellular localizations that had been observed in neonatal rat brain. That is, Pi was found in oligodendrocyte precursors, Mu in astrocytes, and carbonic anhydrase in both oligodendrocyte precursors and astrocytes. In forebrains of 6‐week‐old mice the neurotoxicant, cuprizone, induced oligodendrocyte degeneration, gliosis, and partial demyelinatin. Degeneration of oligodendrocytes, and astrocytosis, began during the initial week of cuprizone feeding, and by the end of the eighth week some demyelination was observed. After mice were fed cuprizone for 4 to 7 weeks, Pi appeared in some of the reactive astrocytes, and Pi‐positive astrocytes were present for at least 7 additional weeks. Normally, Pi appeared only in oligodendrocyts. Very few Pi‐positive oligodendrocyes remained after the second week. During the eighth week healthy‐looking carbonic anhydrase‐positive oligodendrocytes reappeared and began to accumulate, and a few small patches of Pi‐positive oligodendrocytes were also observed. In summary, some novel findings about glial cells were the observation of an enzyme (Pi) that is lost earlier from oligodendrocytes than is carbonic anhydrase, the apparently unique shift in Pi expression from oligodendrocytes to astrocytes and the greater temporal dissociation between loss of oligodendrocytes and demyelination in the older mice. © 1993 Wiley‐Liss, Inc.