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Myelin basic protein mRNA translocation in oligodendrocytes is inhibited by astrocytes in vitro
Author(s) -
AmurUmarjee S.,
Phan T.,
Campagi A. T.
Publication year - 1993
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490360111
Subject(s) - oligodendrocyte , astrocyte , myelin basic protein , myelinogenesis , galactocerebroside , chromosomal translocation , biology , microbiology and biotechnology , messenger rna , neuroglia , myelin , proteolipid protein 1 , glial fibrillary acidic protein , immunology , biochemistry , central nervous system , neuroscience , gene , immunohistochemistry
Myelin basic protein (MBP) mRNAs are translocated from cell bodies into the slender processes connecting oligodendrocyte somas with the myelin sheath in vivo. This translocation was observed in mixed glial cultures prepared from newborn mouse brains and it occurred in approximately 25% of the cells expressing the gene. However, when “enriched” oligodendrocytes were prepared by shaking them free of other glial cells, MBP mRNA translocation occurred into the processes of essentially all of the cells. When enriched oligodendrocytes were plated back onto astrocytes, MBP mRNA was observed to be confined to the cell bodies of almost all the cells, indicating a marketd inhibition of translocation of the mRNA. This inhibition of mRNA translocation did not appear to be mediated through soluble factors secreted by astrocytes or by “astromatrix,” but rather through physical contact between the oligodendrocytes and astrocytes. Intact, but not necessarily live, astrocytes were required for the inhibition of mRNA translocation in the oligodendrocytes. Fibroblasts and a neuroblastoma cell line, SKN‐SH, did not inhibit MBP mRNA translocation in oligodendrocytes suggesting that astrocyte surface‐specific components might be involved in the interaction between astrocytes and oligodendrocytes in culture. These results suggest that contact between these two cell types can influence intramolecular events related to myelinogenesis. © 1993 Wiley‐Liss, Inc.