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Expression of the metabotropic glutamate receptor mGluR1α and the ionotropic glutamate receptor GluR1 in the brain during the postnatal development of normal mouse and in the cerebellum from mutant mice
Author(s) -
Ryo Y.,
Miyawaki A.,
Furuichi T.,
Mikoshiba K.
Publication year - 1993
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490360104
Subject(s) - metabotropic glutamate receptor 1 , cerebellum , metabotropic glutamate receptor , purkinje cell , metabotropic glutamate receptor 6 , biology , metabotropic glutamate receptor 8 , glutamate receptor , granule cell , metabotropic receptor , glutamatergic , metabotropic glutamate receptor 7 , synaptogenesis , microbiology and biotechnology , ionotropic glutamate receptor , ionotropic effect , neuroscience , metabotropic glutamate receptor 5 , receptor , hippocampus , biochemistry , dentate gyrus
Expression of the metabotropic glutamate receptor type 1α (mGluR1α) and the non‐ N ‐methyl‐ D ‐aspartate (NMDA) ionotropic glutamate receptor type 1 (GluR1) in mouse brain was investigated using the antibodies raised against the synthetic peptides corresponding to their C‐terminal amino acid sequences. Both receptor proteins are glycosylated predominantly in an asparagine‐linked manner, and are abundant in post‐synaptic membranes. We showed that mGluR1α and GluR1 expression within the first 3 postnatal weeks undergoes dramatic changes in time and space, i.e., in the hippocampus and cerebellum. These spatio‐temporal expression patterns appear to be correlated with the postnatal ontogenesis and establishment of the glutamatergic neurotransmission system in the hippocampus and cerebellum, cell migration, dendritic and axonal growth, spine formation, and synaptogenesis. In the adult cerebellum, mGluR1α is intensely expressed in Purkinje neurons and GluR1 in Bergmann glial cells. Both receptors are expressed to a fair degree in weaver mutant cerebellum despite granule cell degeneration. However, the intrinsic expression levels of both mGluR1α and GluR1 are markedly reduced in the cerebellum of the Purkinje cell‐deficient and underdeveloped mutant mice, Purkinje ‐ cell ‐ degeneration , Lurcher , and staggerer , suggesting that GluR1 expression in Bergmann glia cells may be correlated with the sustained interaction with adjacent Purkinje neurons. © 1993 Wiley‐Liss, Inc.