Premium
Prednisolone enhances myogenesis and dystrophin‐related protein in skeletal muscle cell cultures from mdx mouse
Author(s) -
Passaquin A. C.,
Metzinger L.,
Léger J. J.,
Warter J.M.,
Poindron P.
Publication year - 1993
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490350403
Subject(s) - myogenesis , dystrophin , duchenne muscular dystrophy , skeletal muscle , mdx mouse , utrophin , endocrinology , myocyte , biology , medicine , microbiology and biotechnology
The differentiation of skeletal muscle cells from mdx mice which lack dystrophin expression was examined after glucocorticoid treatment, namely α‐methylprednisolone (PDN). Primary skeletal muscle cell cultures were established from newborn mdx , congenic C57BL/10, and allogenic BALB/C mice. We show that PDN promotes the myogenesis of both mdx ‐ and control mice‐derived cultures as determined by (1) the number of myotubes, (2) acetylcholine receptors, and (3) dystrophin and dystrophin‐related protein levels. These results support the hypothesis that PDN could enhance the myogenesis of satellite cells and increase dystrophin‐related protein expression in DMD treated patients. © 1993 Wiley‐Liss, Inc.