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110/140 laminin‐binding protein immunoreactivity in spinal dorsal root ganglia: A capsaicin‐insensitive reduction induced by constriction injury of the sciatic nerve in rats
Author(s) -
Ren K.,
Kibbey M. C.,
Kleinman H. K.,
Ruda M. A.
Publication year - 1993
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490350302
Subject(s) - sciatic nerve , constriction , anatomy , neuropathic pain , medicine , nerve injury , dorsal root ganglion , sciatic nerve injury , capsaicin , nociception , anesthesia , dorsum , receptor
The distribution of 110/140 laminin‐binding protein (110/140 LBP) in the spinal dorsal root ganglia (DRG) and its regulation by partial constriction of the sciatic nerve was studied in adult rats. The cross‐sectional area of neurons with 110/140 LBP‐immunoreactivity (−I) showed an approximately normal frequency distribution. The 110/140 LBP‐I was observed in neuronal cell bodies exclusive of the nucleus. Following sciatic nerve constriction, the 110/140 LBP‐I was downregulated in the ipsilateral L4‐5 DRG. DRG neurons with a cross‐sectional area ≥ 1600 μm 2 were preferentially affected. Neonatal capsaicin‐treatment, a procedure that selectively destroys a subpopulation of DRG neurons with fine unmyelinated axons, had no effect on the reduction of 110/140 LBP in the DRG induced by sciatic nerve constriction. Western immunoblot analysis confirmed a reduction of 110/140 LBP on the side ipsilateral to the constriction. These results demonstrate a LBP within primary sensory neurons and its suppression by peripheral nerve injury. The data support a role for LBP in the adult nervous system.© 1993 WiIey‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the Unitcd States oC America.