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Interleukin‐4 and ‐5 as modulators of nerve growth factor synthesis/secretion in astrocytes
Author(s) -
Awatsuji H.,
Furukawa Y.,
Hirota M.,
Murakami Y.,
Nii S.,
Furukawa S.,
Hayashi K.
Publication year - 1993
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490340506
Subject(s) - nerve growth factor , lymphokine , astrocyte , secretion , monoclonal antibody , biology , interleukin , microbiology and biotechnology , endocrinology , medicine , chemistry , antibody , immunology , cytokine , central nervous system , biochemistry , receptor , immune system
To examine the regulation of nerve growth factor (NGF) gene expression with respect to neural trauma, we examined the effects of T cell‐derived lymphokines on NGF synthesis/secretion in cultured mouse astrocytes. Interleukin (IL)‐4 and IL‐5 significantly increased the amount of NGF secreted by astrocytes, whereas IL‐2, IL‐3, and IL‐6 had no significant effect. IL‐4 and IL‐5 produced marked increases in NGF mRNA levels in astrocytes as demonstrated by the reverse transcription‐polymerase chain reaction (RT‐PCR) method. The effect of IL‐4 and IL‐5 was greater in quiescent astrocytes than in growing cells. Neither increase in thymidine incorporation nor any morphological change was observed during the treatment with IL‐4 and IL‐5. The stimulatory effect of IL‐4 and IL‐5 on NGF synthesis was completely inhibited by the addition of anti‐IL‐4 monoclonal antibody and anti‐IL‐5 monoclonal antibody, respectively. The results indicate that IL‐4 and IL‐5 specifically trigger a cascade of events to regulate NGF synthesis in astrocytes, independent of cell growth. © 1993 Wiley‐Liss, Inc.