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Oligodendrocyte differentiation and progenitor cell proliferation are independently regulated by cyclic AMP
Author(s) -
Raible D. W.,
McMorris F. Arthur
Publication year - 1993
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490340305
Subject(s) - microbiology and biotechnology , progenitor cell , oligodendrocyte , progenitor , cell growth , cellular differentiation , biology , chemistry , neuroscience , stem cell , biochemistry , myelin , gene , central nervous system
Oligodendrocytes, the glial cells specialized to synthesize myelin in the central nervous system, differentiate in primary rat brain cell cultures on a schedule similar to that observed in vivo. The schedule of oligodendrocyte differentiation and the rate of oligodendroglial progenitor cell proliferation in vitro are both modulated by 3′,5′‐cyclic AMP (cAMP). A 24‐hour exposure to 1 mM N 6 ,2′O‐dibutyryladenosine 3′,5′‐cyclic monophosphate (dbcAMP) induced a wave of oligodendrocyte differentiation but inhibited proliferation of oligodendroglial progenitors, and reduced by 30‐fold the proliferation of progenitors in response to platelet‐derived growth factor (PDGF). When cells were grown in the presence of maximally stimulating concentrations of PDGF, the inhibitory effect of cAMP on progenitor cell proliferation was abolished while the stimulatory effect of cAMP on oligodendrocyte differentiation remained, demonstrating that these two cAMP‐regulated events are independent. © 1993 Wiley‐Liss, Inc.