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β/A4 domain of APP: Antigenic differences between cell lines
Author(s) -
Wolozin B.,
Zheng B.,
Loren D.,
Lesch K. P.,
Lebovics R. S.,
Lieberburg I.,
Sunderland T.
Publication year - 1992
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490330202
Subject(s) - antigen , domain (mathematical analysis) , computational biology , microbiology and biotechnology , virology , chemistry , biology , immunology , mathematics , mathematical analysis
The expression of amyloid precursor protein (APP) in olfactory neuroblasts has been examined with a panel of antibodies directed against varied regions of the APP molecule. The pattern of reactivity was compared to that in the transformed human glial cell line SVG, human cortical brain tissue, and in kidney epithelial 293 cells containing stably transfected and overexpressed human APP751. Antibodies directed against the C‐terminus and extracellular domains of amyloid precursor protein (APP) react more strongly on immunoblot with transfected 293 cells and brain tissue than with olfactory neuroblasts (ON) or SVG cells. Antibodies directed against the β/A4 region of APP show a contrasting pattern of reactivity, yielding greater reactivity with ON and SVG cells than with transfected 293 cells or brain tissue. Analysis of the APP transcripts using polymerase chain reaction indicates that ON and SVG both make predominantly APP770 and 751, as does the transfected 293 cell line. In the absence of any differences in APP transcripts among the cell lines, the difference in availability of the β/A4 region appears likely to be due to posttranslational modification. These data therfore indicate that processing of APP varies among cell lines and thus may vary from tissue to tissue. © Wiley‐Liss, Inc.