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Nerve growth factor receptor gene expression in human peripheral blood lymphocytes in aging
Author(s) -
Kittur Smita D.,
Song L.,
Endo H.,
Adler W. H.
Publication year - 1992
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490320316
Subject(s) - peripheral blood , nerve growth factor , gene expression , gene , receptor , peripheral , biology , microbiology and biotechnology , cancer research , medicine , immunology , genetics
Nerve growth factor (NGF) has a modulating effect on immune function, which may occur as a consequence of binding to the NGF receptor (NGF‐R). To determine if mRNA for the gene coding for p75 NGFR (low affinity NGF‐R) is present in lymphocytes, Northern blot analysis of mRNA from human peripheral blood lymphocytes (PBL) and purified T lymphocytes was initiated using cDNA probe for human p75 NGFR . p75 NGFR mRNA was present in PBL and T lymphocytes, and the mRNA in response to phytohemagglutinin stimulation showed maximum levels at 14 hr of stimulation. p75 NGFR mRNA content when analyzed in PBL and T cells from volunteers of various ages showed that p75 NGFR mRNA expression does not change with the age of the cell donor. © 1992 Wiley‐Liss, Inc.