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Enhanced permeabilities of cationized‐bovine serum albumins at the blood‐nerve and blood‐brain barriers in awake rats
Author(s) -
Wadhwani Kishena C.,
ShimonHophy M.,
Rapoport S. I.
Publication year - 1992
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490320312
Subject(s) - chemistry , bovine serum albumin , blood–brain barrier , albumin , serum albumin , chromatography , bolus (digestion) , biochemistry , endocrinology , central nervous system , anatomy , biology
Permeability‐surface area products (PAs) of the blood‐nerve barrier (BNB) and blood‐brain barrier (BBB) to 125 I‐labeled native bovine serum albumin (nBSA, pI ∼4), and to 2 cationized albumins (cBSA) of differing pI (pI ∼ 8 and 11), were quantitatively determined in awake rats, using an i.v. bolus injection technique. Mean PAs of the BNB and BBB to 125 I‐nBSA, after a circulation time of up to 120 min, were (0.17 ± 0.23) and (0.09 ± 0.05) × 10 −5 ml/s.g. wet wt, respectively (n =; 12 rats), and were not significantly different from 0 ( P > 0.05). Mean PAs of the BNB and BBB to 125 I‐cBSA (pI ∼ 8), after circulation time of 12 min, were (1.9 ± 0.1) and (1.7 ± 0.1) × 10 −5 ml/s.g wet wt, respectively (n =; 8). Significant greater PAs, at both the BNB and BBB to 125 I‐cBSA (pI ∼ 11) [(8.2 ± 1.8) and (3.0 ± 0.6) × 10 −5 ml/s.g wet wt, respectively (n =; 12)], than both PA's of nBSA and cBSA (pI ∼ 8) were found. The accumulation of 125 I‐cBSA in epi‐perineurial tissues also was higher than that of 125 I‐nBSA, and was related to the degree of cationization. Our results indicate that, as at the BBB, the transfer of cationized serum albumin is enhanced over that of native albumin at the BNB of the mammalian peripheral nerve. © 1992 Wiley‐Liss, Inc.